Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antioxidant and Anti-Cancer Potentials of Rheum emodi Rhizome Extracts

doi: 10.1093/ecam/neq048

Figure Lengend Snippet: IC 50 values of aqueous and methanolic extracts of R. emodi in MDA-MB-435S and Hep3B cell lines as determined in XTT assay.

Article Snippet: MDA-MB-435S (human breast carcinoma) and Hep3B (human liver carcinoma) cell lines were obtained from National Centre for Cell Science (Pune, India).

Techniques:

Journal: BMC Cancer

Article Title: Dysregulated paired related homeobox 1 impacts on hepatocellular carcinoma phenotypes

doi: 10.1186/s12885-021-08637-3

Figure Lengend Snippet: PRRX1 and metabolism of HCC cells. A Glucose consumption and lactate output were measured 48 h after siPRRX1 transfection. Bars indicate mean ± SD and a representative picture of 3 experiments is shown, each in triplicate. B Expression of genes involved in glycolysis were determined by qPCR 48 h after siPRRX1 transfection in HUH7 and HLF cells. Bars indicate mean ± SD and representative of 3 experiments each in triplicates. C Expression of genes involved in the TCA cycle were analyzed by qPCR 48 h after siPRRX1 transfection in HUH7 and HLF cells. Bars indicate mean ± SD and representative of 3 experiments, each in triplicates. D Expression of genes involved in amino acid metabolism as determined by qPCR 48 h after siPRRX1 transfection in HUH7 and HLF cells. Bars indicate mean ± SD and representative of 3 experiments each in triplicates. E Alterations of metabolites. Heatmap of changes in metabolite levels of glycolysis, TCA cycle and amino acids in HUH7 and HLF cells 48 h after siPRRX1 transfection. n = 3 per cell line and condition. White numbers indicate a p < 0.05; black numbers are not significant. The experiment was performed in triplicate

Article Snippet: The cell lines HLF, HUH7, Hep3B and SNU398 were cultured in Dulbecco Modified Eagle’s medium (DMEM, high glucose, Lonza, BE12–709) supplemented with 2 mM glutamine, 10% fetal bovine serum (FBS), penicillin (100 U/ml), and streptomycin (100 μg/ml).

Techniques: Transfection, Expressing

Journal: BMC Cancer

Article Title: Dysregulated paired related homeobox 1 impacts on hepatocellular carcinoma phenotypes

doi: 10.1186/s12885-021-08637-3

Figure Lengend Snippet: Summary of metabolic changes after knockdown of PRRX1 ( A ) in HUH7 and ( B ) in HLF cells. Red indicates upregulation, green indicates downregulation of genes or metabolites, italics: gene expression, bold: dysregulated metabolites. See text for details

Article Snippet: The cell lines HLF, HUH7, Hep3B and SNU398 were cultured in Dulbecco Modified Eagle’s medium (DMEM, high glucose, Lonza, BE12–709) supplemented with 2 mM glutamine, 10% fetal bovine serum (FBS), penicillin (100 U/ml), and streptomycin (100 μg/ml).

Techniques: Knockdown, Gene Expression

Journal: Biomimetics

Article Title: Anticancer Activity of Thiophene Carboxamide Derivatives as CA-4 Biomimetics: Synthesis, Biological Potency, 3D Spheroid Model, and Molecular Dynamics Simulation

doi: 10.3390/biomimetics7040247

Figure Lengend Snippet: IC 50 (µM) of phenyl-thiophene-carboxamide compounds ( 2a – 2e ) on several cell lines.

Article Snippet: Our results showed that the PSA of the most synthesized structures was biomimetic to CA-4, and similar chemical and biological properties were observed against Hep3B cancer cell line.

Techniques:

Journal: Biomimetics

Article Title: Anticancer Activity of Thiophene Carboxamide Derivatives as CA-4 Biomimetics: Synthesis, Biological Potency, 3D Spheroid Model, and Molecular Dynamics Simulation

doi: 10.3390/biomimetics7040247

Figure Lengend Snippet: RHA-3 ( 2b ) and RHA-6 ( 2e ) perturb 3D hepatocellular spheroids’ formation. Images of cluster/s formed by Hep3B hepatocellular carcinoma in presence of 2b (17 µg/mL) or 2e (17 µg/mL) after 24 h of treatment compared to controls ( A ). Cluster percentage of occupied area relative to the negative control ( B ), cluster circularity ( C ), and cluster count ( D ). The non-treated cells are referred to as a negative control. At 100 μg/mL, DOX was utilized as a positive control. The scale bar represents a distance of 10 μm. Circularity scale: a value of 1 represents a perfect circle (ns: p > 0.05, **: p ≤ 0.01, ***: p ≤ 0.001, and ****: p ≤ 0.0001).

Article Snippet: Our results showed that the PSA of the most synthesized structures was biomimetic to CA-4, and similar chemical and biological properties were observed against Hep3B cancer cell line.

Techniques: Negative Control, Positive Control